Journal: Brain, behavior, and immunity

Article Title: Stress disinhibits microglia via down-regulation of CD200R: a mechanism of neuroinflammatory priming

doi: 10.1016/j.bbi.2017.11.001

Figure Lengend Snippet: Primer Sequences.

Article Snippet: mCD200Fc Soluble recombinant mouse CD200 Fc (mCD200Fc) chimera (R & D Systems; cat. no. 3555-CD) and human IgG1Fc (R & D Systems; cat. no. 110-HG) were dissolved in sterile 1x PBS.

Techniques: Sequencing, Activation Assay, Chemotaxis Assay

Journal: Brain, behavior, and immunity

Article Title: Stress disinhibits microglia via down-regulation of CD200R: a mechanism of neuroinflammatory priming

doi: 10.1016/j.bbi.2017.11.001

Figure Lengend Snippet: Animals were injected ICM with control protein (hIgG1Fc) or mCD200Fc. Immediately after injection, animals were exposed to IS or served as HCC. 24h after stress exposure, hippocampal microglia were isolated and treated with LPS for 2h. A) the cytokine response (IL-1β, TNFα and NFkBIα) at each concentration of LPS was captured for the following treatment groups: HCC/hIgG1Fc (N = 8), IS/hIgG1Fc (N = 8), HCC/mCD200Fc (N = 8), and IS/mCD200Fc (N = 7). B) The cumulative cytokine response across concentrations of LPS (area under the curve; AUC) was computed. Data are presented as mean ± sem. In panel A, the effects of stress and mCD200Fc treatment on the cytokine response were analyzed at each concentration of LPS. The cytokine response for the IS/hIgG1Fc group significantly differed from all other treatment groups, ** p < 0.01, *** p < 0.001. In panel B, the cytokine response of the IS/hIgG1Fc group was significantly different from all other experimental groups, *** p < 0.001.

Article Snippet: mCD200Fc Soluble recombinant mouse CD200 Fc (mCD200Fc) chimera (R & D Systems; cat. no. 3555-CD) and human IgG1Fc (R & D Systems; cat. no. 110-HG) were dissolved in sterile 1x PBS.

Techniques: Injection, Control, Isolation, Concentration Assay

Journal: Brain, behavior, and immunity

Article Title: Stress disinhibits microglia via down-regulation of CD200R: a mechanism of neuroinflammatory priming

doi: 10.1016/j.bbi.2017.11.001

Figure Lengend Snippet: (A) Animals were exposed to IS (N = 8) or served as HCCs (N = 8). 24h after IS, hippocampal HMGB1 protein was measured. (B) Animals were injected ICM with control protein (hIgG1Fc) or mCD200Fc. Animals were then exposed to IS or served as HCCs. 24h after stress exposure, hippocampal HMGB1 protein was measured. Data are presented as mean ± sem. For data presented in A, IS significantly differed from HCC, **** p < 0.0001. For data presented in B, the hIgG1Fc/IS treatment group significantly differed from all other treatment groups, ** p < 0.01, *** p < 0.001.

Article Snippet: mCD200Fc Soluble recombinant mouse CD200 Fc (mCD200Fc) chimera (R & D Systems; cat. no. 3555-CD) and human IgG1Fc (R & D Systems; cat. no. 110-HG) were dissolved in sterile 1x PBS.

Techniques: Injection, Control